Please note that single-cell pilot and feasibility projects should be discussed with the participating cores before the application. Our single-cell expert Diana Burkart-Waco, PhD, (firstname.lastname@example.org) will be available for pilot grant project related discussions every day from 3 pm to 5 pm from Thursday, March 14th to Thursday, March 22nd.
To include the other cores please contact Matt Settles (email@example.com ; Bioinformatics) and Bridget McLaughlin (firstname.lastname@example.org; Flow-Cytometry).
The feasibility grant will support the analysis of one sample for the 10X Genomics protocol.
The Office of Research has issued a program supporting pilot and feasibility projects for UC Davis investigators and Campus Research Core Facilities.
For the DNA Technologies Core these funding opportunities will be focusing on single-cell gene expressions studies. Hereby we are collaborating with both the Bioinformatics Core and the Flowcytometry Core.
Single-cell gene expressions studies reveal expression changes that are undetectable via conventional RNA-seq from tissue samples. The latter can only provide transcriptome data averages for the entire sample, confounding transcripts of multiple cell types and cell-cycle stages. In contrast single-cell RNA-seq (scRNA-seq) allows to resolve the cellular heterogeneity.
The DNA Technologies Core offers two protocols for scRNA-seq:
Multi-well plate-based scRNA-seq library prep and sequencing (e.g. after flow-sorting).
Droplet-based high-throughput scRNA-seq library prep and sequencing (using the 10X Genomics system)
- Please inquire with the Flowcytometry Core if your project will involve cell-sorting.
The Bioinformatics Core has lot of experience in single-cell data analysis. Both cores will aim to support Pilot and Feasibility Project Grant applications for their efforts.
- The DNA Technologies Core must provide an estimate for services that you apply for, so please contact email@example.com before you apply to the Campus Program for Pilot and Feasibility Project Grant funds
- For more details please see the call at this page:
- Award preference will be given to projects that will generate long-term use, preliminary data for a new extramural grant or manuscript submissions. Funding from this program will be granted to Academic Senate and Academic Federation members with PI status with the following constraints:
- (1) Who have not used the requested facility in the past 2 years, or
- (2) Investigators who will explore the utilization of equipment and services offered by the facility that they have not used before
- These funds must be utilized within 12 months.
- Please direct administrative questions to firstname.lastname@example.org .
Application deadlines are: March 23; June 15; September 14; & December 14, 2018
For all other types of projects, please use the Genome Center pilot grants or our regular fee-based services.