Which read numbers/yields can I expect from Illumina sequencing?

The Illumina specifications are based on the Illumina PhiX control library. Better or similar yields can be expected for other high complexity libraries (e.g. genomic, RNA-seq libraries) if they are within the recommended insert size ranges and do not average extreme GC-contents. Yields can vary depending on library type. For libraries that fulfill the criteria above, we do promise that the Hiseq 4000 and NextSeq sequencing data  will exceed the Illumina yield specifications.

The table below displays the read numbers as CPF (clusters passing filter). For single-end sequencing CPF is equal to the read numbers. In case of paired-end sequencing the read number is twice the CPF figure.

IlluminaSeqMetrics


Category: 05 Sequencing

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