The table below shows the wide range of outputs from the sequencing platforms currently available in the Core. Illumina and PacBio offer very complementary technologies (Illumina: very high output, but shorter reads; PacBio: long reads at lower throughput). The choice of platform depends on the data requirements for your project – please contact us to discuss your options.
|Sequencing Platforms||HiSeq3000/4000||HiSeq2500 – Rapid Mode||HiSeq2500 – High Output 1||MiSeq||PacBio RS II|
|Sequencing Output per Lane||average 104-110 Gb *||up to 45 Gb||up to 36 Gb 1||up to 15 Gb||500 Mb to 1 Gb (official estimate after titration; potentially up to 1.2 Gb very dependent on library type, DNA quality, library titration)|
|Reads per Lane
in Millions (i.e. Clusters Passing Filter **)
|average 340 -370 M *||up to 170 M||up to 200 M||up to 17 M (v2) up to 25 M (v3)||20-60 K|
|Read Length||up to 2x 150bp||up to 2x 250bp||up to 2x 100bp||up to 2x 300bp||average 9-12kb, N50 up to 17kb|
|Typical Sequencing Format
SR (single reads), PE (paired end reads)
|SR50, PE100, PE150||SR100, PE150, PE250||SR50, PE100 1||SR50, PE75 (v3), PE150, PE250, PE300 (v3)||1-3kb (CCS), 2kb, 5kb, 10kb, 20kb, 30kb|
* The average yield for HiSeq 3000/4000 lanes and customer prepared libraries is 340 million clusters passing filter. For libraries prepared by our facility the average yield is higher at about 370 million clusters. The core-prepared libraries tend to show better size selection ( narrower fragment size range) and adhere closely to the recommendations for the Hiseq3000/4000 libraries and thus tend to cluster more efficiently than the average library.
** The number of “clusters passing filter” is equivalent to the number of “reads passing filter” for single-end sequencing runs. For paired-end sequencing the number of reads is twice the number of clusters according to the way Illumina is counting reads.
1 Due to the much higher read numbers enabled by the Hiseq 3000/4000 sequencers, we usually do not run the Hiseq 2500 in High Output mode anymore. Please inquire with us if you would like to run entire flow-cells..