DNA Technologies Core — Prices 2019

High Throughput Sequencing Service Rates – Per Lane or Cell (prices include qPCR library pool quant. and index reads*)	UC Rate	Non-Profit Rate	Industry Rate
Illumina HiSeq 4000 – SR50 or SR100 Single Read Sequencing; 50 or 100 cycles Illumina Specs: 260-310 million CPF; frequent yields: 320-405 million CPF; average ~375 CPF *******	1127	1753	2156
Illumina HiSeq 4000 – PE100 or PE150 Paired End Sequencing; 2x 100 or 2x 150 cycles Illumina Specs: 260-310 million CPF; frequent yields: 320-405 million CPF; average ~375 CPF *******	2346	3648	4487
Illumina NovaSeq S4 – S4 flowcell lane; PE150; Illumina Specs: 2.5 to 3 billion CPF; 750–900 Gb per lane***	6400 ^a	8064 ^a	8480 ^a
Illumina MiSeq – 50 cycles v2; special order SR50 or PE25 (per run)	987	1535	1888
Illumina MiSeq – 150 cycles v3 SR150 or PE75 (per run)	1067	1659	2041
Illumina MiSeq – 300 cycles v2 SR300 or PE150 (per run)	1208	1878	2311
Illumina MiSeq – 500 cycles v2 PE250 (per run)	1265	1967	2420
Illumina MiSeq – 600 cycles v3 PE300 (per run)	1716	2669	3283
Illumina MiSeq Nano – 300 cycles PE150; special order; Illumina Specs: 1 million CPF***	492 ^a	773 ^a	953 ^a
Illumina MiSeq Nano – 500 cycles PE250; special order; Illumina Specs: 1 million CPF***	557 ^a	875 ^a	1079 ^a
Illumina MiSeq Micro – 300 cycles PE150; special order; Illumina Specs: 4 million CPF***	648 ^a	1017 ^a	1255 ^a
Illumina NextSeq High-Output 75 cycles – PE40 or SE80 Illumina Specs: 330-400 million CPF; >500 million CPF possible *******	1624 ^a	2545 ^a	3140 ^a
Illumina NextSeq High-Output 150 cycles – PE75 or SE150 Illumina Specs: 330-400 million CPF; >500 million CPF possible *******	2965 ^a	4655 ^a	5743 ^a
Illumina NextSeq High-Output 300 cycles – PE150 Illumina Specs: 330-400 million CPF; >500 million CPF possible *******	4654 ^a	7306 ^a	9014 ^a
Illumina NextSeq Mid-Output 150 cycles – PE75 or SE150; Illumina Specs: 108-130 million; >150 million CPF possible *******	1227 ^a	1923 ^a	2376 ^a
Illumina NextSeq Mid-Output 300 cycles – PE150; Illumina Specs: 108-130 million; >150 million CPF possible *******	1873 ^a	2940 ^a	3628 ^a
PacBio Sequencing of 1st Sequel SMRT cell V3 (new chemistry) – per library: 10hr run ; 20hr LR run	1344 ; 1516 ^a	2110 ; 2380 ^a	2603 ; 2937 ^a
PacBio Sequencing of a subsequent Sequel SMRT cells V3 (new chemistry) – per library: 10hr run ; 20hr LR run	1172 ; 1348 ^a	1840 ; 2116 ^a	2270 ; 2611 ^a
Oxford Nanopore PromethION Sequencing and library prep for the 1st flowcell; larger projects: please inquire	2880 ^a	3629 ^a	please inquire

* All Illumina sequencing runs include the sequencing of up to two index reads up to 8bp in length and demultiplexing. Please contact dnatech@ucdavis.edu.
** In case you want to sequence infrequently used run-types (e.g. SE150) we would require you to use all 8 lanes of the HiSeq flow cell with your samples. Please inquire.
*** CPF: Clusters passing filter. Each cluster generates one forward and, for paired-end sequencing only, one reverse read. The yield metrics apply to standard balanced libraries like RNA-seq or genomic libraries.
^aPreliminary rates (pending approval). Will be billed as “special consumables”.

NEW: Illumina Sequencing Packages (including library preparation and sequencing)	UC Rate	Non-Profit Rate	Industry Rate
Whole Genome Shotgun Sequencing – library prep and PE150 bp sequencing to 30X human genome coverage (96 Gb for other genomes)	1000 ^a	1260 ^a	1325 ^a
Human Exome Sequencing – library prep, enrichment, and PE150 bp sequencing 80x coverage; 39 Mb target; minimum 8 samples	200 ^a	252 ^a	265 ^a
Batch-Tag-Seq (RNA-Seq) – 3’Tag-Seq Gene Expression Profiling both library prep & sequencing; minimum 8 samples	104 ^a	163 ^a	201 ^a
Batch-Tag-Seq (RNA-Seq) & Data Analysis – Gene Expression Profiling & Data Analysis library prep & sequencing & DGE analysis; minimum 8 samples; in part billed by Bioinformatics	184 ^b	289 ^b	355 ^b

Library Services* and HMW-DNA Preparation (price per library including Library QC; sample QC provided by client or at additional cost through us)	UC Rate	Non-Profit Rate	Industry Rate
Genomic DNA Sequencing Library Preps (HT) requires samples at normalized concentrations)	80	124	153
NEW: SHT Pooled DNA Library Preps (per 96 normalized DNA samples in 96-well plate)	960 — $10/sample ^a	1507 — $15.70/sample ^a	1853 — $19.30/sample ^a
RNA-Seq Library Preps; Poly-A Enriched (HT) starting from 6, 12, or 24 libraries respectively	170 ; 130 ; 100	265 ; 203 ; 156	325 ; 249 ; 192
Ribo-depleted RNA (HT) – Ribo-depletion and strand-specific library prep	180	279	344
NEW: 3’Tag-Seq (RNA-Seq) – gene expression profiling – e.g. Quant-Seq library preps only	80 ^a	126 ^a	155 ^a
NEW: Batch-Tag-Seq (RNA-Seq) – 3’Tag-Seq gene expression profiling – library prep & sequencing (minimum 8 samples)	104 ^a	163 ^a	201 ^a
NEW: Single-Cell RNA-Seq – 10X Genomics scRNA-seq library preps; up to 8 samples per day	1x $231 + $1836 per sample ^a	1x $363 + $2883 per sample ^a	1x $448 + $93 per sample ^a
Ultralow Input RNA-seq – Clontech SMART-Seq kit	262 ^a	411 ^a	507 ^a
micro RNA – miRNA-seq library prep including size-selection — up to 48 samples	1x $670 + $93 per sample ^a	1x $1052 + $146 per sample ^a	1x $1298 + $3557 per sample ^a
WGBS METHYL-seq Library Prep – whole-genome shotgun bisulfite seq	149 ^a	234 ^a	288 ^a
RRBS METHYL-seq Library Prep — per 12 samples	1292 ^a	2028 ^a	2502 ^a
RR-Seq Library Prep – reduced representation, per 95 samples, plated	1450	2255	2774
NEW: 10X Chromium Genome Linked Read Library Preps; 10X Genomics	918 ^a	1441 ^a	1778 ^a
NEW: HMW DNA Isolation- for 10X, PacBio, and Nanopore sequencing; up to 4 samples (animal samples)	1x $594 + $100 per sample ^a	1x $932 + $157 per sample ^a	1x $1150 + $194 per sample ^a
Mate-Pair Library Prep, Gel-Free – including one or two tagmentation reactions respectively; for low inputs; without MP size selection	450 ; 650	700 ; 1011	861 ; 1243
Mate-Pair Library Prep, Gel-Plus – including one or two tagmentation reactions respectively; with MP size selection	1450 ; 1750	2255 ; 2721	2774 ; 3348
Library Pooling (Qubit Quantification) – up to 6 samples	62	96	118
PacBio Express Library Prep w. size selection	426 ^a	669 ^a	826 ^a
PacBio Express Double-Library-Prep w. size selection	621 ^a	975 ^a	1203 ^a
PacBio SMRT Library Prep for most applications use Express library preps instead	470	731	899
PacBio SMRT Library Prep HT or amplicon – 6 or more libraries	345	536	660
PacBio Iso-Seq Library Prep — full length RNA-Seq — options for pooled preps; inquire	770 ^a	1208 ^a	1491 ^a
Blue Pippin Size Selection – up to 4 samples (PacBio), 5 samples (other)	137	213	262
Hi-C Library Preparation – first and second samples, each	1690 ^a	2129 ^a	please inquire
Hi-C Library Preparation – samples three to eight, each	574 ^a	723 ^a	please inquire
RNA-Seq Data Analysis (Bioinformatics Core) & incl. gene count tables & simple comparison DGE analyses
DGE Data Analysis Only for RNA-Seq, 3′-Tag-Seq, performed and billed by (minimum 12 samples, requires reference genome and GTF gene annotation data)	80 — technical info	125 — technical info	154 — technical info
DNA/RNA & Library Quantification and QC Services	UC Rate	Non-Profit Rate	Industry Rate
DNA Quantification (Qubit) up to 12 samples	62	96	118
Pico-Green DNA Quantification on Plate Reader up to 96 samples	144	224	275
Library Quantification (qPCR) per sample	23	36	44
Bioanalyzer Library QC per sample, 1-3 samples	34	53	65
Bioanalyzer HS DNA & Small RNA chips per set of 4-11 samples	118	184	226
Bioanalyzer DNA, RNA Pico & Nano chips up to 11 or 12 samples	98	152	187
Weekly RNA-QC on LabChip Gx Wednesdays: 2 sample minimum; cost per sample	5 ^a	7.80 ^a	9.70 ^a
LabChip Gx QC – up to 48 samples	246 ^a	333 ^a	471 ^a
LabChip Gx QC – up to 96 samples	398	617	761
Fluidigm EP1 System Genotyping	UC Rate	Non-Profit Rate	Industry Rate
STA Pre-Amp per 192.24 IFC (chip)	281	437	537
Assay Processing per IFC (48.48, 96.96 or 192.24 chip)	464	721	887
Illumina Infinium II Genotyping – Microarrays	UC Rate	Non-Profit Rate	Industry Rate
Assay Processing: 4 Chips same format	1088	1692	2081
Assay Processing: 8 Chips same format	1368	2128	2617
Miscellaneous	UC Rate	Non-Profit Rate	Industry Rate
Instrumentation Training**	109	170	209
Equipment Use Fee per hour	20	32	39
Agilent Bioanalyzer Supplies per HS DNA or Small RNA chip	56	87	107
Agilent Bioanalyzer Supplies per RNA Nano/Pico/DNA 12k chip	36	56	69
LabChipGX Supplies per set of 48 samples	148	231	284
Blue Pippin Cartridge/Supplies	60	94	115
Labor Rate per hour	62	96	118

* Please consult the Sequencing and Sequencing Library pages for more details on these services. All library preparation and package services include library QC and pooling. The sample QC can be provided by the client or will be carried out at additional cost by the Core. Additional services related to library normalization, bar-coding, and other manipulations are available – please contact (dnatech@ucdavis.edu). Fluidigm EP1 System genotyping prices are based on a combination of charges 1) Fluidigm materials and reagents, which will vary depending on the format and scale of the project, and 2) IFC chip assay processing costs (shown above). For detailed pricing information contact Vanessa Rashbrook VKRashbrook@ucdavis.eduInfinium genotyping prices are based on a combination of charges 1) Illumina materials & reagents and 2) chip processing costs. Please contact Vanessa Rashbrook VKRashbrook@ucdavis.edu for a quote.
** More information on training found at this site. Non-UCD users must submit a PO (customer name, institution, description of work, approx. $ amount, and PO#) before services can be initiated. In case of questions, please contact Jen Stevens at our Business Office Administration (JenStevens@ucdavis.edu). We now also accept credit cards. Prices current as of August 30th, 2017.
^aPreliminary rates (pending approval). Will be billed as “special consumables”.
^bPreliminary rates (pending approval). Will be billed as “special consumables”. $80 of these per sample will be billed by Bioinformatics Core.

The Genome Center Cores provide services at three recharge rate scales. The University of California rate scale (UC rates) do apply to all projects paid from UC-system accounts. Non-profit/academic rates do apply to all other non-profit research including government projects. The industry rates apply to all projects paid from for-profit businesses or institutions. No taxes will apply to these rates.