Blog Archives

RNA-Seq Workshop – Library Preparation and Introduction to Data Analysis – August 9th to 11th 2017

August 9th, 9 a.m. – August  11th,  5 p.m. RNA-seq has revolutionized the way we address complex biological questions, allowing for differential gene expression, differential transcript analysis, as well as transcriptome assemblies. As sequencing output rapidly increases and sample numbers increase, library

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Another Update on the Barcode Mis-Assignment Issue: Illumina’s White Paper

Illumina published a white paper today on the barcode mis-assignment issue (Illumina calls it somewhat euphemistically “barcode hopping”). In contrast to the Sinha et al. paper, which only presented data for libraries with NextSeq adapter sequences, the white paper shows that

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Update on Barcode Mis-Assignment Issue

A “much-tweeted” manuscript was recently published on Biorxiv, investigating the barcode mis-assignment issues affecting HiSeq 3000, 4000, and HiSeq X, reported in our last newsletter.  Please see the manuscript here: Sinha et al. 2017  http://biorxiv.org/content/early/2017/04/09/125724 . As mentioned previously in our newsletter, the

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April News: High-Throughput Gene-Expression Analysis – Index mis-assignments on HiSeq – NextSeq sequencing – Pippin HT library size selection

New High-Throughput Gene-Expression Analysis Services (3’Tag-Seq) The DNA Technologies and the Bioinformatics Cores have been working for more than half a year with a simplified RNA-Seq protocol that promises to drastically reduce the costs of many gene-expression studies, while fully maintaining the

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PacBio Sequel Version 2 Chemistry and new Iso-Seq Protocol

PacBio Sequel Version 2 Chemistry   We have recently beta-tested the new PacBio Sequel chemistry (V2).  The tests have demonstrated significant yield and read-length improvements.  All current and future Sequel runs will be carried out with the new chemistry.  In

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Open between the holidays

We will be open before, after, and between the holidays and closed only on official UC holidays. The UC holidays are Friday the 23rd, Monday the 26th, Friday the 30th, and Monday the 2nd. Happy Holidays! The DNA Tech Core

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Seminar: Simultaneous “multi-omic” measurement of gene fusions, mRNA, & proteins at 800-plex using single-molecule optical barcodes

Please joins us for the seminar: Simultaneous “multi-omic” measurement of gene fusions, mRNA, & proteins at 800-plex using single-molecule optical barcodes. Thursday, December 1st:  2pm to 3 pm Genome Center Auditorium (GBSF, #1005) Refreshments provided. Please RSVP using this link. ABSTRACT The ability

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New Service: Long-Read Sequencing on the PacBio Sequel

Pacific Biosciences (PacBio) has recently published a high quality de novo genome assembly for Arabidopsis based on the data of only two Sequel SMRT-cells. The Sequel is the second generation PacBio sequencer and generates up to 7x more reads per

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RNA-Seq Workshop – Library Preparation and Introduction to Data Analysis – February 8th to 10th 2017

February 8th, 9 a.m. – February  10th,  5 p.m. RNA-seq has revolutionized the way we address complex biological questions, allowing for differential gene expression, differential transcript analysis, as well as transcriptome assemblies. As sequencing output rapidly increases and sample numbers increase, library preparation

Posted in Courses, News

RNA-Seq Workshop – Library Preparation and Introduction to Data Analysis – November 15th to 17th

We are sorry the workshop is fully booked. To subscribe to the our email list which announces the workshops, send an email to sympa@ucdavis.edu with the following information in the subject line: subscribe dnatech_news first_name last_name November 15, 2016, 9 a.m. – November 17,

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