DNA Technologies Core

DNA_logo_bannerThe mission of the DNA Technologies Core at the UC Davis Genome Center is to provide DNA analytical services to campus and other researchers.  We are currently focused on Next-Gen Sequencing (NGS) and genotyping, using mainly Illumina (HiSeq 3000, HiSeq 2500 and MiSeq), PacBio (RSII), and Fluidigm (C1-single cell analysis and Access Array) platforms. Our goal is to enable access to high throughput genome-wide analyses at economical recharge rates, as a functional extension of your laboratory. The services offered by the Genome Center complement other UC Davis facilities in order to provide access to numerous technologies for genomic analysis.

Read more about Getting Started in the Cores.

Services and Equipment

 

Technology Platforms Facility Services
Illumina Sequencing HiSeq 3000, HiSeq 2500 & MiSeq
PacBio Sequencing PacBio RSII
Fluidigm C1 Single Cell Genomics Auto-Prep
Fluidigm Access Array Target Amplification
Infinium Genotyping
Fluidigm EP1 Genotyping
Next-Gen Sequencing (Illumina and PacBio)
Next-Gen Library Prep
DNA Quantification/Library QC
Single Cell Genomics
Genotyping
Expression Analysis
Equipment: Training & Access
Sample QC: Agilent Bioanalyzer, Nanodrop, Caliper LabChip GX
Sample Prep: Covaris E220, Diagenode Bioruptor & Bioruptor NGS, Sage BluePippin
Automated Library Prep: Caliper Sciclone G3, IntegenX Apollo 324, Fluidigm Access Array

News

  • PacBio Sequel Version 2 Chemistry and new Iso-Seq Protocol

    March 9, 2017
    PacBio Sequel Version 2 Chemistry   We have recently beta-tested the new PacBio Sequel chemistry (V2).  The tests have demonstrated significant yield and read-length improvements.  All current and future Sequel runs will be carried out with the new chemistry.  In addition to the V2 chemistry change, the Sequel software has been updated and allows now for 10 ...
  • Open between the holidays

    December 15, 2016
    We will be open before, after, and between the holidays and closed only on official UC holidays. The UC holidays are Friday the 23rd, Monday the 26th, Friday the 30th, and Monday the 2nd. Happy Holidays! The DNA Tech Core Staff.
  • Seminar: Simultaneous “multi-omic” measurement of gene fusions, mRNA, & proteins at 800-plex using single-molecule optical barcodes

    November 11, 2016
    Please joins us for the seminar: Simultaneous “multi-omic” measurement of gene fusions, mRNA, & proteins at 800-plex using single-molecule optical barcodes. Thursday, December 1st:  2pm to 3 pm Genome Center Auditorium (GBSF, #1005) Refreshments provided. Please RSVP using this link. ABSTRACT The ability to measure changes to DNA, RNA, and protein is crucial to developing a comprehensive understanding of biology in the genomics era. NanoString ...
  • New Service: Long-Read Sequencing on the PacBio Sequel

    November 10, 2016
    Pacific Biosciences (PacBio) has recently published a high quality de novo genome assembly for Arabidopsis based on the data of only two Sequel SMRT-cells. The Sequel is the second generation PacBio sequencer and generates up to 7x more reads per SMRT-cell compared to the first generation PacBio RSII sequencer. The DNA Technologies Core is now ...
  • RNA-Seq Workshop – Library Preparation and Introduction to Data Analysis – February 8th to 10th 2017

    October 27, 2016
    February 8th, 9 a.m. – February  10th,  5 p.m. RNA-seq has revolutionized the way we address complex biological questions, allowing for differential gene expression, differential transcript analysis, as well as transcriptome assemblies. As sequencing output rapidly increases and sample numbers increase, library preparation becomes one of the major bottlenecks. This workshop provides comprehensive hands-on training in the preparation of ...
  • RNA-Seq Workshop – Library Preparation and Introduction to Data Analysis – November 15th to 17th

    September 27, 2016
    We are sorry the workshop is fully booked. To subscribe to the our email list which announces the workshops, send an email to sympa@ucdavis.edu with the following information in the subject line: subscribe dnatech_news first_name last_name November 15, 2016, 9 a.m. – November 17, 9 a.m. to 6 p.m. RNA-seq has revolutionized the way we address complex biological questions, allowing for differential gene ...
  • RNA-Seq Workshop – Library Preparation and Introduction to Data Analysis – May 10th to 12th

    April 9, 2016
    May 10, 2016, 9 a.m. – May 12, 2016, 9 a.m. to 6 p.m. Please see this page for the full information and the registration:  https://registration.genomecenter.ucdavis.edu/events/DNATech_RNASeq_May_2016/ RNA-seq has revolutionized the way we address complex biological questions, allowing for differential gene expression, differential transcript analysis, as well as transcriptome assemblies. As sequencing output rapidly increases and sample numbers increase, ...
  • We are a Campus Research Core Facility!

    March 25, 2016
    The UC Davis DNA Technologies and Expression Analysis Cores have been designated a Campus Research Core Facility From the official announcement: ” …. This designation provides a strategic platform to ensure state-of-the-art research capabilities provided in a cost-efficient manner. The designated laboratories are expected to develop and represent best practices for core facility operation and emerge as centers of excellence for ...
  • DNA Technologies Core User Survey Results

    March 25, 2016
    We would like to thank everybody who participated in our user survey! Your input is highly appreciated and will help us improve our services. The results are available here: https://www.surveymonkey.com/results/SM-Q67N6H9J/ 73 DNA Technologies and Expression Analysis Core users did participate.
  • HiSeq3000 upgraded to Hiseq4000

    February 10, 2016
    Our latest generation Illumina HiSeq3000 sequencer has been upgraded to a HiSeq4000.  This means the option to run two flowcells in parallel was enabled.  Thus, the sequencer offers now twice the throughput.  The chemistry and reagents do not change as should the yields per lane and the high quality of the data.