Monthly Archives: March 2017

PacBio Sequel Version 2 Chemistry and new Iso-Seq Protocol

PacBio Sequel Version 2 Chemistry   We have recently beta-tested the new PacBio Sequel chemistry (V2).  The tests have demonstrated significant yield and read-length improvements.  All current and future Sequel runs will be carried out with the new chemistry.  In

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  • Fascinating data on the gut microbiome and associations with depression. The Raes lab and others are tweeting thei… ,
  • Good single-cell RNA-seq (scRNA-seq) depends on starting with "happy", viable cells and the absence of cell debris.… ,
  • Low input IsoSeq preps are not a problem at the expense of a view additional PCR cycles. ,
  • Variation across 141,456 human exomes and genomes reveals the spectrum of loss-of-function intolerance across human… ,
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