The Qubit 2 Fluorometer allows precise quantification of DNA and RNA samples from minimum sample volumes. Since the fluorescence signals are generated after binding of DNA- or RNA-specific dyes, the assay tolerates a lot of contaminants. We recommend carrying out all DNA or RNA sample and library quantification by fluorometry instead of spectrometry, as the latter is highly unreliable. The Qubit measurements are carried out in individual 0.5 ml optically clear tubes, typically measuring 1 ul sample volumes. Larger sample volumes of up to 10 ul can be used.
For larger sample numbers we recommend fluorometric quantification on the plate reader.
We offer the following Qubit assays:
RNA High-Sensitivity: quantification range 5 to 100 ng RNA Broad-Range: quantification range 20 to 1000 ng dsDNA High-sensitivity quantification range 0.2 to 100 ng
ThermoFisher also offers Qubit assays for protein and miRNA quantification.