Sample Submission, Scheduling & Shipping

Sample Submission   (libraries, DNA or RNA samples for library preps or QC)

  • Please make sure that your lab has an account with the Genome Center and that you have the required billing information  (see the “Getting Started” page).
  • Verify that your samples fulfill all the requirements.
  • We must receive electronic and print copies of the submission forms in order to receive and schedule your samples. Please download the forms from the following links.
SAMPLE SUBMISSION FORMS:
Illumina Sequencing:
– Submitting your libraries for HiSeq or MiSeq sequencing:   Sequencing Submission Form
– Submitting DNA samples for library prep and sequencing:  DNA Library Prep & Sequencing Submission Form
– Submitting RNA samples for library prep and sequencing:  RNA Library Prep & Sequencing Submission Form
PacBio Sequencing:
– Samples for PacBio sequencing:                                          PacBio Submission Form
10X Genomics:
– Samples for 10X Genomics Chromium Genome prep and seq:    10X Chromium Genome Submission Form
Sample QC and Other Services:
– Nucleic Acid or library QC (Bioanalyzer, Caliper Gx, Qubit, etc.) & Megaruptor requests: QC Submission Form

 

  • Fill in the information electronically, email it to dnatech@ucdavis.edu and print a copy to accompany your samples. We can only accept samples if we receive a hardcopy of your submission form together with them!
  • We require Bioanalyzer traces (or similar) to work with customer submitted sequencing libraries as well as for total RNA samples. If no traces are submitted with the libraries/samples, we will carry out the Bioanalyzer QC for a fee. If you can supply traces, attach them to email and/or include them into the shipment in hard copy.
    Please make sure that the samples conform to the sample or library requirements: see this page.
  • Submit your samples in 1.5 ml microcentrifuge tubes (clearly labeled on the lid). We suggest using low-retention tubes (e.g. Eppendorf LoBind). Please protect these tubes in a sturdy box or in disposable 50 ml centrifuge tubes from damage from cold-packs. If using wet ice, double-wrap your samples. Please use unique sample names!
  • DNA samples for large scale projects can also be submitted in sealed 96-well PCR plates.   To ensure that your samples arrive without leakage and cross-contamination, use one of the following types of seals: –Heat Seal (this is the preferred method. Please use a removable heat seal that allows the plate to be resealed such as “Easy Peel”) or adhesive aluminum foil seals like “AlumaSeal” . Protect the plates  in a sturdy box. Sample shipments of plates should be carried out on a surplus of frozen “blue ice” blocks or on dry ice,  to insure that the samples remain frozen at all times
  • For genomic DNA and other dsDNA shipments in tubes, cold packs (e.g. “blue ice”) are usually sufficient. For RNA dry ice is recommended. Please only ship with courier services (FedEx, UPS, DHL). If you are shipping DNA for PacBio libraries, please follow the PacBio guidelines for shipping and handling.
  • Do not ship any samples before a weekend (i.e., on a Thursday for Friday arrival) or the day before a holiday: see this calendar of our University holidays.
  • Please use the following shipping address and ship samples via courier services (FedEx, UPS, DHL) :
Lutz Froenicke/Sample Submission
DNA Technologies Core
Genome and Biomedical Sciences Facility (GBSF), Room 1410
University of California-Davis
451 Health Sciences Drive
Davis, CA 95616
Phone: 530-754-9143

For international shipments: UC Davis EIN-number # 94-6036494

Please contact us if you have any questions about the required information. If you drop off your samples in person we can go over the submission form with you. It is essential that you fill in all the appropriate information to minimize the chance for error on these expensive and time consuming experiments. If we are not in the lab when you come by, please deposit your samples in the white polystyrene cool box on the lab bench – do not deposit them in the under-counter freezer or remove any samples from this freezer.

Demultiplexing – It is important to ensure your sample IDs and barcodes are unique – we are now implementing additional labor charges for re-demultiplexing because of incorrect submission info, duplicate sample names, wrong barcodes given, reverse complements given in error, etc.

Scheduling

The turnaround times can be difficult to predict far in advance, since it depends on the requested options and the queue length at time of sample drop off. Thus we can usually give a better estimate when your sample is delivered. For MiSeq runs the turnaround time is typically five to eight days, while you should allow four to six weeks for the HiSeq sequencers. Please see our HiSeq Calendar & Queue for the projected HiSeq sequencing schedules. We do encourage scheduling discussions at the outset of your project since, depending on the requirements of your run, we may need to order particular reagents. This is particularly true for non-standard ribo-depletion requests and some MiSeq runs as the 50 and 300 cycle kits are not kept in stock and need to be special ordered.