Two new sequencers have been installed in the Genome Center, both deliver highest read accuracies: The PacBio REVIO long-read sequencer and the Element Biosciences AVITI short-read sequencer.
- The PacBio REVIO reduces sequencing cost by two-thirds and increases the HiFi long-read sequencing capacity 7.5 times compared to its two predecessors. Thus, the HiFi read technology now becomes much more affordable and will enable the generation of pan-genome data including structural genomic variation. As before, these highest accuracy long-read data will be suited for de-novo genome assemblies and full-length transcript sequencing (Iso-seq).
- HiFi reads will have lengths of 15 kb to 18 kb and mean read accuracies of 99.95% (Q33) for high-quality DNA samples.
- The HiFi base-calling now makes use of Google Health Deep Consensus algorithms to further increase accuracies.
- Methylation calling is included in all genome sequencing data. Sequencing of a single SMRT-cell will be sufficient to de novo assemble a human genome.
Learn more about the REVIO here: https://www.pacb.com/revio/
The REVIO will be introduced during a big Launch Party
on April 12th in the Genome Center:
https://dnatech.genomecenter.ucdavis.edu/2023/04/05/pacbio-revio-launch-party-on-march-12th/
The Element Biosciences AVITI sequencer is introduced here.
