We carry out a lot of 16S sequencing – almost daily – with customer prepared samples.
If you generate barcoded amplicons yourself we will be happy to add Illumina adapters (required by some protocols) and sequence them on our MiSeqs.
We don’t generate 16S libraries in-house, because UC Davis has a facility specializing in 16S analysis: The Host Microbe Biology Systems Core (HSMBC; also located in our building). The HSMBC offers a complete service ranging from DNA isolation, 16S amplification, sequencing (performed in the DNA Tech Core), to data analysis. You can certainly also pick selected parts of their service that you require. Please contact the HSMBC manager for details.