Consultations
We can provide better advice on appropriate or optimal sequencing options if you provide us important background information on your project. We suggest using the questions below as a starting point for inquiries.
Information requested for RNA-sequencing projects:
- Which species are you studying (prokaryotic or eukaryotic)?
- From which sample types will the RNA be isolated (e.g. tissues, cell cultures, whole organisms)?
- What is the objective of the study (e.g. differential gene-expression (DGE) study, gene annotation, identification of iso-forms)?
- For differential DGE studies: Are you interested only in gene-level DGE, or do you want to study the expression of the different transcripts of genes? Are you interested in non-coding RNAs?
- Do you expect lowly expressed genes to become important for your study?
- How closely are related (genetically) are the biological replicates?
- Do you expect to induce strong phenotypic changes by the varying treatments?
- What total RNA amounts do you expect to extract from the samples?
- What is the expected integrity of the RNA samples (e.g. RIN-scores)? Do you expect potentially interfering chemicals in the samples (e.g. phytochemicals)?
- How many samples do you want to study? Will the samples be available at the same time?
RNA-seq options are described on two pages: RNA-seq and 3′ Tag-Seq Gene Expression Profiling.
Information requested for DNA-sequencing projects:
- Which species are you studying (prokaryotic or eukaryotic)?
- What type of samples are you going to submit (e.g. genomic DNA, amplicons, metagenomic samples, purified strain or population samples)?
- What is the expected genome size? What genome coverage are you aiming for?
- What is the objective of the study (e.g. SNV variant analysis, genotyping, de novo genome assembly, structural genome variants, metagenomic analysis)?
- For de novo genome assemblies: Are you interested in genic regions or do you require the full genome sequence? Do you require the ordering of the assembly scaffolds?
- What DNA amounts do you expect to extract from the samples?
- What is the expected DNA sample integrity (fragment sizes)? Do you expect potentially interfering chemicals in the samples (e.g. phytochemicals)? What protocol are you using for the DNA isolation?
- How many samples do you want to study?
Most DNA-Seq options are described here.
In-Progress Project Inquiries
To be able to help you quickly with current or recent projects we always need the following information:
- PI name
- Submitter name
- Project ID (e.g. HiSeq3421)
- Submission date (even if vague)
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