SNP genotyping options for small to medium scale projects are now available with the recent acquisition to the Core of the Fluidigm EP1 System. This platform is an alternative to the Illumina BeadXpress/Golden Gate Assay genotyping platform. The EP1 System offers the most efficient sample/SNP genotyping throughput using end-point reading after PCR (check out the Fluidigm genotyping web page for more information on the technology). It allows for low operating costs and provides the easiest workflow for low- to mid-multiplex SNP genotyping. More details on the Core service options will be posted here when available.
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- Short-Read Sequencing Services – Illumina & Element Bio AVITI
- DNA Sequencing (whole-genome shotgun, targeted, amplicon, exome, ChIP, reduced-representation, Methyl, …)
- RNA-Sequencing (high-throughput mRNA-Seq, total RNA-seq, 3′-Tag-Seq, miRNA-seq, … )
- Gene Expression Profiling with 3′-Tag-Seq
- PacBio Revio Library Prep & Sequencing
- Nanopore Sequencing – PromethION
- Linked Read Sequencing – 10X Genomics Chromium Technology
- High Molecular Weight DNA Isolation (HMW-DNA)
- Sample and Library Requirements Tables
- DNA/RNA Quantification & Library QC
- DNA and RNA Extraction at the Taqman Core
- Single Cell Expression Profiling & Genomics (10X Genomics, Parse Biosciences, and Plate-based scRNA-Seq)
- Spatial Transcriptomics
- Genotyping – Illumina Infinium, Fluidigm EP1
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- Booking Shared Instruments – Equipment Calendar
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- Agilent Bioanalyzer 2100
- FilterMax F5 Plate Reader
- Qubit Fluorometer
- Caliper LabChip GX (high-throughput Bioanalyzer)
- Nanodrop Spectrophotometer
- Sonicators
- BluePippin and PippinHT
- Robotic Liquid Handlers
- Fluidigm C1 – Single Cell Genomics
- Fluidigm Access Array
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