The sample amount requirements are chosen to ensure both, high-quality data and efficient processing.
Most of the library prep protocols will generate sequenceable libraries with lower input amounts than request, often requiring additional PCR cycles.
Processing often low-input samples often requires additional handling and QC steps. Thus, additional custom processing costs may apply.
Please contact us before submitting such samples. When working with sample amounts lower than recommended, you will generally run the risk of introducing biases and noise into your data. This may or may not be an acceptable trade-off for your specific project.
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