Can you run samples with less than the recommended input material?

The sample amount requirements are chosen to ensure both, high-quality data and efficient processing.
Most of the library prep protocols will generate sequenceable libraries with lower input amounts than request, often requiring additional PCR cycles.
Processing often low-input samples often requires additional handling and QC steps. Thus, additional custom processing costs may apply.

Please contact us before submitting such samples. When working with sample amounts lower than recommended, you will generally run the risk of introducing biases and noise into your data. This may or may not be an acceptable trade-off for your specific project.

Category: 03 Sample Preparation & Sample Requirements

Posted in
Latest Tweets
  • Ipipet is/was (?) a very handy tool for aiding manual pipetting in and out of 96-well plates - using tablets as a g… ,
  • The IntelliKin works with array tapes - just like our Intelliqubes. qPCR for 4x 768 samples in a smaller form fact… ,
  • PCR-like DNA amplification with an enzyme cocktail that can be heat-killed. Looks very promising. SHARP amplificati… ,