Agilent Bioanalyzer 2100

bioanalyzerThe Agilent Bioanalyzer uses a microchannel-based electrophoretic cell that allows rapid and sensitive investigation of nucleic acid samples. Many sample types can be analyzed on the Bioanalyzer, including total RNA, labeled RNA, small and micro RNAs, as well as small and large DNA fragments.

The following kits are kept in stock by the Core:
RNA chips – good for measuring RNA integrity, assessing labeled RNA or fragmented RNA size distribution, or polyA+ purification efficacy.  Not recommended for accurate quantification. Several typical examples of total RNA profiles run on the Bioanalyzer can be found at this Ambion hosted web site.
     RNA Pico Kit: 0.1 – 5 ng/ul sensitivity. Up to 11 samples/chip
     RNA 6000 Nano Kit: 25-500 ng/ul sensitivity. Up to 12 samples/chip.

DNA chips – good for Size QC, not recommended for accurate quantification.
DNA 12000 Kit: 5-50 ng/ul sensitivity, size range 100-12000 bp. Up to 12 samples/chip.
DNA High Sensitivity Kit: 0.5-3 ng/ul sensitivity, 50-7000 bp size range. Up to 11 samples/chip.

For information on other kits that are available to purchase, please consult the Agilent Bioanalyzer site.  You are welcome to use any of these products on our instrument following appropriate training.

Using the Bioanalyzer

Users may drop off samples to be run on the Bioanalyzer (see the DNA Quantification/Library QC page) or become trained users. Use of the Bioanalyzer conforms to the standard guidelines for equipment usage in the Core (for information visit the Training and Use of Core Facility Equipment page).  Additionally, you must have a Genome Center account to use the instrument, which you can create here if necessary.

If you have already completed a formal training with facility personnel, please use the on-line calendar to reserve a 1 hour time slot during normal working hours. Put your name in the “subject” box, and in the “description” box please put phone number, DaFIS account, type of assay to be run, and laboratory PI. Please bring your own pipettes (0.5-10 ul volumes are used), tips, gloves, and ice bucket (we can provide ice).  For RNA chips, we recommend bringing your samples over as 2 uL aliquots in 0.5 mL tubes for the denaturing step. Condensed instruction sheets summarizing the protocol for the DNA HS and RNA Nano kits are available and should be consulted before starting your run; among other things this document will further explain what you need to bring and what we provide.

Bioanalyzer Output

The software that runs the Bioanalyzer has a number of useful analytic options. It is available on the computer that runs the instrument, but because the Bioanalyzer is in fairly constant use it is usually not feasible to do much analysis there. We have another copy of the software on a Core workstation, which you’re free to use if available. A demo version of the software is available from Agilent; you can do everything the normal software does except save or export your file. Typically we create a pdf of the file once it is fully analyzed, then put this on our web server for you to download locally.