How to prepare samples for multiplexed amplicon sequencing on Illumina systems?

There are multiple valid protocols available for amplicon sequencing on Illumina systems.  Here we describe one of many options: A  two-step PCR protocol to generate complete sequencing libraries. This protocol has the advantage that it does not require custom sequencing primers

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Which protocols or kits do you recommend for RNA isolations from human and animal samples? How many cells will I need?

Many types of RNA-seq require RNA samples of high integrity and high chemical purity – please see the sample requirements.  If the tissue or cell samples are handled correctly (e.g. flash frozen and stored at -80C) standard spin column RNA extraction

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How do I ship RNA samples? How do I ship RNA samples if the transport will take a long time?

RNA samples should best be shipped on dry ice. Please only ship with courier services (FedEx, UPS, DHL). For longer transports (e.g. from South America) we also had very good success with RNA samples shipped dry at room temperature (after

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How do I prepare DNA samples for RR-Seq (reduced representation sequencing)

Sample preparation: If it has been established that a restriction enzyme (e.g. ApeKI) and method are suitable for the species you are working on (please see below), we require the samples for RR-Seq to be submitted in a 96-well plate.  One

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Do you offer DNA isolations and RNA isolations as a service?

At the moment we only carry out high-molecular-weight DNA  (HMW-DNA) isolations for the purpose of 10X Genomics and Nanopore sequencing.  Please inquire with Ruta Sahasrabudhe, PhD. We do not offer DNA isolations for Illumina sequencing and RNA isolations at the moment.  However the Taqman Core

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How to prepare samples for multiplexed amplicon sequencing on the PacBio Sequel?

The best option for sequencing larger numbers of amplicons that exceed the MiSeq read length limitations (e.g. amplicons longer than 600 nt) is Pacbio sequencing. Amplicons up to 12 kb length can generate highest quality sequence data for individual molecules employing circular-consensus-sequencing (CCS) analysis. 

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Do you have recommendations for the isolation of plant total RNA samples?

The isolation of high-quality DNA and RNA samples from plants can be challenging due to the presence of inhibiting and damaging phytochemicals.  Thus, it is not possible to recommend a single protocol that works for all samples.   In any case

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How should I prepare and sequence samples for ChIP-seq?

If we prepare the sequencing libraries we require ChIP-seq DNA samples to be submitted after reversal of the cross-linking. Ideally, the fragment lengths should be between 100 and 300 bp, and preferably under 500 bp. The former will result in

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How should I purify my samples? How should I remove DNA or RNA contamination?

Bead based sample cleanups (e.g., Ampure XP, RNAClean XP) and spin column-based protocols (e.g., Qiagen, Zymo, NorgenBiotek) tend to be the most efficient ways to remove chemical contaminants. For genomic DNA samples to be sequenced on Illumina sequencers, we suggest spin

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What are the sample requirements for DNA and RNA samples or for sequencing libraries ?

Quality and quantity of DNA and RNA is critical for high quality sequencing output. Please make sure your DNA is not degraded and is free of RNA contamination. RNA samples should always be assessed on the bioanalyzer for the absence

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